Confocal POM
| Make: | Carl Zeiss |
| Model: | Axioimager M1m |
| Year of purchase: | 2008 |
| Funded by: | |
| Location: | Arkavathi |
| Contact email: | psantra@cens.res.in |
| Book New Request | |
- Microscope :
- Fully Motorized polarizing optical microscope for transmitted and reflected light studies
- •Light Sources:
- -Halogen lamp 12 V/100 W
- -Mercury vapor lamp HBO 100 W; HBO 50 W with fibre-optic coupling
- •Motorized bright-field condenser module
- •Independently rotatable polarizer and analyzer (with high extinction ratio) in both transmission and reflection geometries.
- •Calibrated rotational sample stage; resolution: 0.1 o with Vernier scale
- •X-Y direction movement: 75 mm x 50 mm; resolution: 0.1 mm
- •Z-axis positioning:
- -coarse and fine focusing wheels (manual movement);
- -motorized drive with step size: 25 nm.
- •Objectives (working distance in parentheses):
- -Plan-Apochromat 63X/1.40 Oil (0.19 mm);
- -Epiplan-Neofluar 50X/0.8 (0.58 mm);
- -LD Epiplan-Neofluar 50X/0.55 (9 mm);
- -Epiplan-Neofluar 20X/0.5 (2.1 mm);
- -LD Epiplan-Neofluar 20X/0.22 (12.1 mm);
- -LD Epiplan 20X/0.4 (7.2 mm);
- -Epiplan 10X/0.2 (18.4 mm);
- -Epiplan-Neofluar 10X/0.25 (9.3 mm);
- -Epiplan-Neofluar 5X/0.13 (15.8 mm).
- •Eye pieces give an additional 10x magnification
- •Conoscopy module (for imaging the back focal plane of objective) with Bertrand lens
- •Aperture and field diaphragm control for transmitted / reflected light
- •Compensators: Tilting K 0-30λ; tilting 0-5λ; QUARTZ Wedge 0-4λ; λ and λ/4 plates
- •LCD touch screen to control the microscope
- •Confocal imaging:
- -LSM 5 Laser Scanning Microscope Exciter
- -Sources: Multiline Ar laser 458/488/514 (25 mW), He-Ne laser (1 mW)
- -Detectors: single channel PMT detector in reflection; Transmitted light photodetector.
- -Fluorescence filters
- Imaging
- •High-resolution digital imaging with AxioCam MRc5 Camera
- •CCD- Peltier cooling
- •5 MP sensor; 2584 x 1936 resolution;
- •36-bit colour depth; pixel size: 3.4 μm x 3.4 μm
- •Image capture rate: 16 fps max;
- •Direct and full control of the microscope with Axiovision software.
- •Image correction during live imaging for eg: hue, gamma and brightness control;
- •Intergration time: 1 ms to 60 s; time-lapse imaging
- Temperature controller
- •Hotstage: Instec HCS402 with Controller STC200
- •Temperature range: Ambient (~ 25 °C) to 300 °C with 0.1 °C resolution
- •Heating rate: min: 0.1 °C/ hr; max: 150 °C/ min (@ 100 °C)
- •Cooling rate: min: 0.1 °C/ hr; max: 50 °C/ min (@ 100 °C)
- •Sample chamber size: 50 mm (L) x 38 mm (W) x 2 mm (H);
- -Height extendable upto 8 mm
- •Manual x-y sample positioning inside the chamber with 10 μm resolution
- •Sample viewing aperture: 2mm dia for transmitted light; 10 mm dia for reflected light
- Regular microscopy
- •Polarizing optical microscopy in Transmission/ reflection modes
- •Hotstage microscopy
- •Conoscopy mode
- •Birefringence studies with tilting compensator (both B and K type)
- Confocal microscopy *
- •Laser Confocal imaging
- •Laser scanning transmission imaging
- •Fluorescence imaging
- •Imaging the molecular alignment and density distribution of fluorescent samples
- (* currently not available)
| Modes | Duration (hrs) |
|---|---|
| Confocal/ fluorescence microscopy * | - |
| Regular microscopy: -Transmission / reflection / conoscopy | User defined |
| Sl. No. - Category | Fees ( in Rs/- ) + additional 18% GST |
|---|---|
| 01 - Academic Institutes | 2,500 per slot / 4hrs |
| 03 - External Industry Users | 5,000 per slot / 4hrs |
| 02 - External Industry Partner | 3,750 per slot / 4hrs |