Confocal POM
Confocal POM
Make: Carl Zeiss
Model: Axioimager M1m
Year of purchase: 2008
Funded by:
Location: Arkavathi
Contact email:
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  • Microscope :
  • Fully Motorized polarizing optical microscope for transmitted and reflected light studies
  • •Light Sources:
  • -Halogen lamp 12 V/100 W
  • -Mercury vapor lamp HBO 100 W; HBO 50 W with fibre-optic coupling
  • •Motorized bright-field condenser module
  • •Independently rotatable polarizer and analyzer (with high extinction ratio) in both transmission and reflection geometries.
  • •Calibrated rotational sample stage; resolution: 0.1 o with Vernier scale
  • •X-Y direction movement: 75 mm x 50 mm; resolution: 0.1 mm
  • •Z-axis positioning:
  • -coarse and fine focusing wheels (manual movement);
  • -motorized drive with step size: 25 nm.
  • •Objectives (working distance in parentheses):
  • -Plan-Apochromat 63X/1.40 Oil (0.19 mm);
  • -Epiplan-Neofluar 50X/0.8 (0.58 mm);
  • -LD Epiplan-Neofluar 50X/0.55 (9 mm);
  • -Epiplan-Neofluar 20X/0.5 (2.1 mm);
  • -LD Epiplan-Neofluar 20X/0.22 (12.1 mm);
  • -LD Epiplan 20X/0.4 (7.2 mm);
  • -Epiplan 10X/0.2 (18.4 mm);
  • -Epiplan-Neofluar 10X/0.25 (9.3 mm);
  • -Epiplan-Neofluar 5X/0.13 (15.8 mm).
  • •Eye pieces give an additional 10x magnification
  • •Conoscopy module (for imaging the back focal plane of objective) with Bertrand lens
  • •Aperture and field diaphragm control for transmitted / reflected light
  • •Compensators: Tilting K 0-30λ; tilting 0-5λ; QUARTZ Wedge 0-4λ; λ and λ/4 plates
  • •LCD touch screen to control the microscope
  • •Confocal imaging:
  • -LSM 5 Laser Scanning Microscope Exciter
  • -Sources: Multiline Ar laser 458/488/514 (25 mW), He-Ne laser (1 mW)
  • -Detectors: single channel PMT detector in reflection; Transmitted light photodetector.
  • -Fluorescence filters
  • Imaging
  • •High-resolution digital imaging with AxioCam MRc5 Camera
  • •CCD- Peltier cooling
  • •5 MP sensor; 2584 x 1936 resolution;
  • •36-bit colour depth; pixel size: 3.4 μm x 3.4 μm
  • •Image capture rate: 16 fps max;
  • •Direct and full control of the microscope with Axiovision software.
  • •Image correction during live imaging for eg: hue, gamma and brightness control;
  • •Intergration time: 1 ms to 60 s; time-lapse imaging
  • Temperature controller
  • •Hotstage: Instec HCS402 with Controller STC200
  • •Temperature range: Ambient (~ 25 °C) to 300 °C with 0.1 °C resolution
  • •Heating rate: min: 0.1 °C/ hr; max: 150 °C/ min (@ 100 °C)
  • •Cooling rate: min: 0.1 °C/ hr; max: 50 °C/ min (@ 100 °C)
  • •Sample chamber size: 50 mm (L) x 38 mm (W) x 2 mm (H);
  • -Height extendable upto 8 mm
  • •Manual x-y sample positioning inside the chamber with 10 μm resolution
  • •Sample viewing aperture: 2mm dia for transmitted light; 10 mm dia for reflected light
  • Regular microscopy
  • •Polarizing optical microscopy in Transmission/ reflection modes
  • •Hotstage microscopy
  • •Conoscopy mode
  • •Birefringence studies with tilting compensator (both B and K type)
  • Confocal microscopy *
  • •Laser Confocal imaging
  • •Laser scanning transmission imaging
  • •Fluorescence imaging
  • •Imaging the molecular alignment and density distribution of fluorescent samples
  • (* currently not available)
Modes Duration (hrs)
Confocal/ fluorescence microscopy * -
Regular microscopy: -Transmission / reflection / conoscopy User defined
Sl. No. - Category Fees ( in Rs/- ) + additional 18% GST